BULLOUS PEMPHIGOID ANTIGEN .2. ISOLATION FROM THE URINE OF A PATIENT

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (2) , 605-608
Abstract
Bullous pemphigoid antigen [Ag] was isolated from the nondialyzable urinary components of a patient with the disease. The isolation was accomplished by ion exchange chromatography and gel filtration. Pemphigoid Ag was a basic glycoprotein that on SDS gel electrophoresis showed 2 major bands, 1 in the 18,000 MW region and the 2nd with a MW of 74,000. Between these 2 bands, 2 additional bands appeared; 1 of 35,000 daltons and the other of 68,000 daltons. The 18,000 MW band was eluted from the gel and rerun on SDS gels. These gels showed the 18,000 MW band and the appearance of the 35,000 and 74,000 MW bands. Urinary pemphigoid Ag may exist as a single monomeric form and in polymeric aggregates.

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