Use of ion exchange chromatography for the study of RecA‐DNA interaction
- 25 February 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 279 (2) , 270-272
- https://doi.org/10.1016/0014-5793(91)80165-y
Abstract
In vitro binding or RecA protein to double-stranded DNA (dsDNA) was studied using ion-exchange liquid chromatography. The method allowed quantification of both free DNA and free protein. The results unambiguously showed a binding stoichiometry or 3 base pairs per RecA monomer. The binding exhibited cooperativity, and the stoichiometry suggested that RecA does not form complexes with two molecules or dsDNA. More than 90% of RecA molecules in the sample were active for DNA binding.Keywords
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