Nuclear Detection of Cellular Retinoic Acid Binding Proteins I and II with New Antibodies
Open Access
- 1 October 1998
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 46 (10) , 1103-1111
- https://doi.org/10.1177/002215549804601002
Abstract
Apart from the retinoic acid nuclear receptor family, there are two low molecular weight (15 kD) cellular retinoic acid binding proteins, named CRABPI and II. Mouse monoclonal and rabbit polyclonal antibodies were raised against these proteins by using as antigens either synthetic peptides corresponding to amino acid sequences unique to CRABPI or CRABPII, or purified CRABP proteins expressed in E. coli. Antibodies specific for mouse and/or human CRABPI and CRABPII were obtained and characterized by immunocytochemistry and immunoblotting. They allowed the detection not only of CRABPI but also of CRABPII in both nuclear and cytosolic extracts from transfected COS-1 cells, mouse embryos, and various cell lines.Keywords
This publication has 27 references indexed in Scilit:
- Analysis of the Effects of CRABP I Expression on the RA-Induced Transcription Mediated by Retinoid ReceptorsBiochemistry, 1996
- The RXR heterodimers and orphan receptorsPublished by Elsevier ,1995
- Nonsteroid nuclear receptors: What Are genetic studies telling us about their role in real life?Cell, 1995
- Crystal structures of cellular retinoic acid binding proteins I and II in complex with all-trans-retinoic acid and a synthetic retinoidStructure, 1994
- Localization of CRABP‐I and CRABP‐II mRNA in the early mouse embryo by whole‐mount in situ hybridization: Implications for teratogenesis and neural developmentDevelopmental Dynamics, 1994
- All-trans and 9-cis retinoic acid induction of CRABPII transcription is mediated by RAR-RXR heterodimers bound to DR1 and DR2 repeated motifsCell, 1992
- Immunodetection of multiple species of retinoic acid receptor α: Evidence for phosphorylationExperimental Cell Research, 1992
- Domains of cellular retinoic acid-binding protein I (CRABP I) expression in the hindbrain and neural crest of the mouse embryoMechanisms of Development, 1992
- Retinoic acid receptor gamma: specific immunodetection and phosphorylation.The Journal of cell biology, 1991
- CRABP and the teratogenic effects of retinoidsTrends in Genetics, 1991