Cold‐tolerant (psychrotrophic) moulds and blue stain fungi from softwood in Sweden. Growth rates in relation to pH and temperature

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Abstract
Seventy isolates of moulds and blue stain fungi (Zygomycota and Deuteromycota), isolated from discoloured outdoor softwood in Sweden, comprising of 27 different species, (the two largest genera Penicillium and Cladosporium) were investigated for their linear growth at three different start‐pH values (5, 7 and 9.5) at two temperatures (2°C and 24°C) on malt extract agar (MEA). At 24°C all isolates showed growth at all three start‐pH values except for one isolate which did not grow at initial‐pH 9.5. After 21 days at 2°C at the three start pH‐values, only six isolates showed no growth indicating that 64 of the isolates were cold‐tolerant (psychrotrophic). Of these 64 strains, 58 showed growth at an initial pH of 9.5. Lower pH optima at 2°C than at 24°C were found for most of the isolates. The reduction of the linear growth at initial pH 9.5 in relation to the growth at optimal pH was more pronounced (higher) at the low temperature.

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