High-Resolution Capillary Isoelectric Focusing of Complex Protein Mixtures from Lysates of Microorganisms
- 22 October 1999
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 71 (23) , 5348-5353
- https://doi.org/10.1021/ac9909305
Abstract
High-resolution capillary isoelectric focusing separations of complex protein mixtures have been obtained for cellular lysates of Saccharomyces cerevisiae, Eschericia coli, and Deinococcus radiodurans. High quality separations are shown to be achievable for total protein concentrations of I) difference of 0.004 are shown to be separated using a linear carrier ampholyte (linear pH gradient between two electrodes) of 3−10. Approximately 45 discrete peaks in the pI range of 5−7 were obtained for S. cerevisiae, ∼80 peaks in the pI range of 4.5−8.5 for E. coli, and ∼210 peaks in the pI range of 3−8.8 for D. radiodurans.Keywords
This publication has 8 references indexed in Scilit:
- High Throughput Proteome-Wide Precision Measurements of Protein Expression Using Mass SpectrometryJournal of the American Chemical Society, 1999
- Probing Proteomes Using Capillary Isoelectric Focusing-Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass SpectrometryAnalytical Chemistry, 1999
- '98 Escherichia coli SWISS‐2DPAGE database updateElectrophoresis, 1998
- Capillary isoelectric focusing: the problem of protein solubilityJournal of Chromatography A, 1997
- Capillary isoelectric focusingElectrophoresis, 1997
- Experimental verification of parameters calculated with the statistical model of overlap from chromatograms of a synthetic multicomponent mixtureAnalytical Chemistry, 1990
- Adaptation of the equipment for high-performance electrophoresis to isoelectric focusingJournal of Chromatography A, 1985
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976