Tumor-Specific Antigen Solubilized by Hypertonic Potassium Chloride

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Abstract
Water-soluble, tumor-specific antigens were obtained from diethylnitrosamine-induced guinea-pig hepatoma cell lines by extraction of lyophilized tumor cells with 3 M KCI. The delayed cutaneous hypersensitivity reaction was used to measure tumor-antigen activity. Under optimal conditions, 15–40% of the antigenic activity present on the viable tumor cells was recovered in soluble form. The immunologic specificity of two antigenically unrelated cell lines was preserved in the soluble extracts. The high yield and stability of the active material enabled us to achieve partial purification of the antigens by standard methods of protein fractionation.

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