The isolation of osmotic-remedial conditional lethal mutants of Candida albicans
- 1 April 1990
- journal article
- research article
- Published by Springer Nature in Current Genetics
- Vol. 17 (4) , 293-296
- https://doi.org/10.1007/bf00314875
Abstract
We have developed a method for the isolation of a broad range of conditional lethal mutants of the pathogenic fungus Candida albicans. The method substantially alleviates the problems posed by the diploid nature, and the biased mutant spectrum, exhibited by most strains of this organism. We have used the method to isolate 560 temperature-sensitive mutants which grew at 30°C but not at 42°C. We identified amongst them a number of osmotic-remedial strains which, at the restrictive temperature, show phenotypes indicating defects in cell wall biosynthesis.This publication has 16 references indexed in Scilit:
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