A method for demonstrating the efficacy of interventions designed to limit infarct size following coronary occlusion: beneficial effect of hyaluronidase

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Abstract
In order to measure the protective effect of interventions following coronary artery occlusions in dogs, the creatine kinase activity of myocardial tissue was assayed after 24 h and related to the myocardial blood flow of that tissue measured with 85Sr labelled microspheres injected 15 min after occlusion. This assay showed normal levels when flow exceeded 50 cm3·min−1·100 g−1. In myocardium with flow reduced to 0 to 15 cm3·min−1·100 g−1, creatine kinase activity was 7.6 ± 0.6 IU·mg−1 protein in control dogs and 13.1 ± 1.8 IU·mg−1 protein (P−1 of hyaluronidase 20 min after occlusion. Where myocardial blood flow was reduced to 16 to 50cm3·min−1·100 g−1, creatine kinase activity was increased from 14.1 ± 1.1 to 20.5 ± 1.4 IU·mg−1 protein by hyaluronidase. This method therefore assesses ischaemic damage independent of electrophysiological measurements and confirms myocardial preservation by hyaluronidase.