STUDIES WITH INAGGLUTINABLE ERYTHROCYTE COUNTS. I. A METHOD FOR MEASUREMENT OF NET GAIN OR DEFICIT OF RED CELLS IN THE HUMAN SUBJECT

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Abstract
Human subjects of blood group A or B were transfused with group O blood and those belonging to type N were given type M blood. Frequent accurate counts of the transfused cells were made after agglutinating the recipient''s erythrocytes with appropriate anti-A, anti-B, or anti-M serum, according to a modification of Ashby''s method. Simultaneously counts of the recipient''s cells were obtained by measuring the concn. of total cells and subtracting those of the donor. The donor''s cells were demonstrated to disappear from the circulation in a rectilinear fashion when plotted on Cartesian coordinates. Using the observed counts of transfused cells as biologic tags, a ratio between recipient''s cells and donor''s cells could then be predicted for any time during the studies. Significant observed deviations from the predicted ratio could then be employed to measure net gain or deficit of the recipient''s erythrocytes. Equations were derived for proper calculation. Detailed examples of clinical studies were presented. The standard error of measurement of erythrocyte counts was computed and equations were derived for the standard error of computed values employed. Data are presented to show that the counts of cells vary with shifts in the volume of plasma of the recipient and these are accounted for in the equations. By this procedure it is possible to measure percentile gains and deficits of the patient''s erythrocytes over periods as long as 120 days (the avg. survival time of normal transfused cells ).
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