Assay of Defective-interfering Semliki Forest Virus by the Inhibition of Synthesis of Virus-specified RNAs
- 1 June 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 54 (2) , 273-280
- https://doi.org/10.1099/0022-1317-54-2-273
Abstract
A simple, rapid and reproducible assay for defective-interfering Semliki Forest virus (DI SFV), which is based on the inhibition of synthesis of virus-specified RNA in SFV-infected cells, is described. Using the assay, it was shown that DI virus is generated by a single passage in baby hamster kidney (BHK) cells in an inoculum which contained no detectable DI virus. The UV target size of the interfering activity was calculated.This publication has 8 references indexed in Scilit:
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