The Specificity of Antibodies to Factor VIII Produced in the Rabbit after Immunization with Human Cryoprecipitate

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Abstract
The relationship between the activity-neutralizing and precipitating activities of rabbit antibody to human factor VIII was studied by measuring the changes in levels of the two activities in two sensitized rabbits after stimulation with cryoprecipitates prepared from the plasmas of normal subjects, haemophiliacs and patients with von Wil- lebrand’s disease. Injection of cryoprecipitates prepared from plasmas with a detectable level of factor VIII clotting activity was followed by a marked rise in the level of activity-neutralizing antibody, whereas there was no rise or a continued fall in level after injection of cryoprecipitates prepared from haemophilic plasmas without detectable factor VIII activity. The level of precipitating antibody rose markedly after injection of normal and haemophilic cryoprecipitates, but little or not at all after cryoprecipitates prepared from the plasma of the patients with von Willebrand’s disease. It is suggested that the specific antigenic sites associated with factor VIII clotting activity were not present in cryoprecipitates prepared from haemophilic plasmas without detectable factor VIII activity, and also that antibodies of two different specificities could be detected.

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