Comparative metabolism of histamine in two amphibia anura

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Abstract
The metabolism of histamine has been compared inRana ridibunda andDiscoglossus pictus, both Amphibia anura. An intralymphatic injection of14C-histamine (5 μCi) was administered to the animals and the total radioactivity excreted in the urine was measured 24, 48, and 72 hours later. The metabolites excreted in the urine at 24 hours were analyzed by thin-layer chromatography and subsequent autoradiography. In both species approximately 60–70% of the injected dose was found in the urine by 72 hours. InRana ridibunda two histamine metabolites were found: imidazoleacetic and methylimidazoleacetic acid. The present results suggest that the catabolism of histamine differs in the two species.