Lysosomal Hydrolases in Neutrophils, Mononuclear Cells and Serum during Escherichia coli Endotoxinaemia in Pigs

Publisher Website
Google Scholar
Abstract
The appearance of 10 different lysosomal enzymes in leukocytes and serum were studied during various stages of E. coli endotoxemia in pigs. The animals were monitored in halothane anesthesia and ventilated with a mechanical ventilator. The endotoxin infusion induced a marked increase in pulmonary artery pressure while the systemic blood pressure was significantly lowered. A pronounced decline in circulating leukocytes occurred. There was a general trend of intracellular reduction of lysosomal enzymes in both polymorphonuclear leukocytes and mononuclear cells; increasing serum levels of the same enzymes occurred. Significantly higher serum levels of all lysosomal enzymes were found 5 h after start of endotoxin infusion compared to preinfusion values, while a significant acidosis in the aorta blood was registered. Evidently, lysosomal enzymes are released from white blood cells following endotoxin stimulation. When local pH values allow enzyme activity, they may contribute to the tissue destruction seen in endotoxemia.

This publication has 15 references indexed in Scilit: