Keratinocyte-specific modulation of type VII collagen gene expression by pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-1beta)
- 1 April 2005
- journal article
- Published by Wiley in Experimental Dermatology
- Vol. 14 (4) , 289-294
- https://doi.org/10.1111/j.0906-6705.2005.00316.x
Abstract
Previous studies have shown that pro-inflammatory cytokines such as tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta up-regulate type VII collagen gene (COL7A1) expression in cultured dermal fibroblasts. The present study was designed to investigate the effects of TNF-alpha and IL-1beta on COL7A1 expression in epidermal keratinocytes. We demonstrated that both TNF-alpha and IL-1beta reduced COL7A1 expression in epidermal keratinocytes in an additive manner, whereas they increased COL7A1 expression in dermal fibroblasts. Thus, regulation of COL7A1 by pro-inflammatory cytokines is cell type specific. In particular, the inhibitory effects of TNF-alpha and IL-1beta occurred, at least in part, at the transcriptional level. Finally, we demonstrated that TNF-alpha and IL-1beta enhanced the TGF-beta-mediated up-regulation of COL7A1 expression in HaCaT keratinocytes, suggesting that the combination of TGF-beta and TNF-alpha or IL-1beta induces a signaling pathway that is completely different from that induced by either pro-inflammatory cytokine alone.Keywords
This publication has 26 references indexed in Scilit:
- Scarless Wound HealingPublished by Taylor & Francis ,2000
- α2β1 Integrin Mediates Dermal Fibroblast Attachment to Type VII Collagen via a 158-Amino-Acid Segment of the NC1 DomainExperimental Cell Research, 1999
- Wound Healing--Aiming for Perfect Skin RegenerationScience, 1997
- Dominant dystrophic epidermolysis bullosa: identification of a Gly-->Ser substitution in the triple-helical domain of type VII collagen.Proceedings of the National Academy of Sciences, 1994
- Type VII Collagen, Anchoring Fibrils, and Epidermolysis BullosaJournal of Investigative Dermatology, 1993
- A missense mutation in type VII collagen in two affected siblings with recessive dystrophic epidermolysis bullosaNature Genetics, 1993
- Molecular biology and pathology of type VII collagen*Experimental Dermatology, 1992
- Type VII collagen gene expression by cultured human cells and in fetal skin. Abundant mRNA and protein levels in epidermal keratinocytes.Journal of Clinical Investigation, 1992
- Burn wounds resurfaced by cultured epidermal autografts show abnormal reconstitution of anchoring fibrilsJAMA, 1988
- Type VII collagen is a major structural component of anchoring fibrils.The Journal of cell biology, 1986