A Bacillus subtilis Gene of Previously Unknown Function, yhaG , Is Translationally Regulated by Tryptophan-Activated TRAP and Appears To Be Involved in Tryptophan Transport
- 15 April 2000
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (8) , 2329-2331
- https://doi.org/10.1128/jb.182.8.2329-2331.2000
Abstract
Computer analysis of the Bacillus subtilis genome sequence revealed a gene with no previously attributed function, yhaG , specifying a transcript containing a presumptive binding site for the tryptophan-activated regulatory protein, TRAP. The presumptive TRAP binding site overlaps the yhaG Shine-Dalgarno sequence and translation initiation region. TRAP was shown to regulate expression of yhaG translationally. Production of the yhaG transcript in vivo was found to compete for the binding of TRAP to other known TRAP binding sites. YhaG is likely to be a transmembrane protein involved in tryptophan transport.Keywords
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