Time-Based Changes in Fibroblast Three-Dimensional Locomotory Characteristics and Phenotypes

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Abstract
Three-dimensional loçomotory trajectories have been determined for an embryonic fibroblast population and also for these cells after a 20-day period in tissue culture (C-20 cells). Differences in loçomotory characteristics between these two cell populations are reported. Applying factor analysis to the distribution of angle changes between vectors in a cell’s trajectory reveals the possibility that different loçomotory phenotypes exist. Of the three phenotypes detected in the embryonic population only one continues in the C-20 population while a new phenotype appears in the latter. These results document changes in loçomotory characteristics and phenotypes in an embryonic population with time.

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