On Malyl-coenzyme A Synthetase

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Abstract
Malyl-CoA synthetase (L-malate: CoA ligase (ADP)) which specifically catalyzed the malyl-CoA formation from L-malate and CoA in the expense of ATP was isolated and partially purified from cell-free extracts of Rhodopseudomonas spheroides. The enzyme also catalyzed a rapid ATP-Pi exchange which was strictly dependent upon the presence of L-malatc and CoA. Also Mg++ was required for these reactions. Reversibility of the reaction of malyl-CoA synthesis was established by employing ADP, Pi and chemically synthesized DL-malyl-CoA which has been proved to have the hydroxyl group at βpostion.