Activation of Sphingosine Kinase by the Bradykinin B2 Receptor and Its Implication in Regulation of the ERK/MAP Kinase Pathway
- 6 January 2001
- journal article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 382 (1) , 135-9
- https://doi.org/10.1515/bc.2001.020
Abstract
Sphingosine kinase phosphorylates sphingosine to generate sphingosine 1-phosphate, a phospholipid that has been implicated in signaling by a number of transmembrane receptors and was recently shown to act as a ligand for a specific class of G protein-coupled receptors. Here we show that the G protein-coupled bradykinin B2 receptor activates sphingosine kinase leading to a time- and dose-dependent elevation of cellular sphingosine 1-phosphate levels that was blocked by the sphingosine kinase inhibitor dihydrosphingosine. Furthermore, increasing doses of this inhibitor partially affected the bradykinin-mediated ERK/MAP kinase activation and fully blocked the protein kinase C-independent component of the signaling pathway from the B2 receptor to the ERK/MAP kinase cascade. Overexpression of sphingosine kinase did not additionally increase the bradykinin-induced ERK/MAP kinase activity, indicating a permissive rather than activating role of sphingosine 1-phosphate in B2 receptor-mediated mitogenic signaling.Keywords
This publication has 26 references indexed in Scilit:
- G Protein-Coupled Receptor-Mediated Mitogen-Activated Protein Kinase Activation through Cooperation of Gαq and Gαi SignalsMolecular and Cellular Biology, 2000
- Bradykinin B2 Receptor-Mediated Mitogen-Activated Protein Kinase Activation in COS-7 Cells Requires Dual Signaling via Both Protein Kinase C Pathway and Epidermal Growth Factor Receptor TransactivationMolecular and Cellular Biology, 1999
- Formyl Peptide Receptor Signaling in HL-60 Cells through Sphingosine KinaseJournal of Biological Chemistry, 1999
- A Subfamily of G Protein-Coupled Cellular Receptors for Lysophospholipids and LysosphingolipidsPublished by Springer Nature ,1999
- A Novel Mitogenic Signaling Pathway of Bradykinin in the Human Colon Carcinoma Cell Line SW-480 Involves Sequential Activation of a Gq/11 Protein, Phosphatidylinositol 3-Kinase β, and Protein Kinase CεJournal of Biological Chemistry, 1998
- Lipid biochemistry: functions of glycerolipids and sphingolipids in cellular signalingThe FASEB Journal, 1997
- A role for Pyk2 and Src in linking G-protein-coupled receptors with MAP kinase activationNature, 1996
- Signal-transducing protein phosphorylation cascades mediated by Ras/Rho proteins in the mammalian cell: the potential for multiplex signallingBiochemical Journal, 1996
- Role of transactivation of the EGF receptor in signalling by G-protein-coupled receptorsNature, 1996
- Intracellular Calcium Release Mediated by Sphingosine Derivatives Generated in CellsScience, 1990