An unusual 5' splice sequence is efficiently utilized in vivo.
- 1 May 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (9) , 2733-2737
- https://doi.org/10.1073/pnas.81.9.2733
Abstract
The minor adult chicken alpha-globin gene (alpha D) has an intron splicing sequence at the 5' end of the second intron that begins with the dinucleotide G-C rather than the usual G-T. To understand what role this splice sequence plays in the processing and maturation of nuclear RNA to cytoplasmic RNA, we have analyzed the intron processing of the alpha D-globin transcript in both heterologous (monkey) and homologous (chicken) cells using simian virus 40 and retrovirus vectors, respectively. In both cell types, both introns of the alpha D-globin gene are efficiently and precisely removed.This publication has 34 references indexed in Scilit:
- Alternative RNA splicing of the murine αA-crystallin gene: Protein-coding information within an intronCell, 1983
- Corrections to the nucleotide sequence of the src gene of Rous sarcoma virusNature, 1983
- Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globin geneNature, 1983
- Binding sites of viral protein P19 onto Rous sarcoma virus RNA and possible controls of viral functionsJournal of Molecular Biology, 1982
- Linkage of β-thalassaemia mutations and β-globin gene polymorphisms with DNA polymorphisms in human β-globin gene clusterNature, 1982
- Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice siteNature, 1982
- Chromosomal arrangement of the chicken β-type globin genesCell, 1981
- SV40-transformed simian cells support the replication of early SV40 mutantsCell, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977