A Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–RFLP) Assay for the Discrimination of Mitochondrial DNA from the Florida and Northern Subspecies of Largemouth Bass

Abstract
Distinct mitochondrial DNA haplotypes, based on the presence or absence of a Taq I restriction site in the control region, distinguished largemouth bass Micropterus salmoides of the northern subspecies (N = 62) from the Florida subspecies (N = 28). The presence of either northern (N = 6) or Florida (N = 3) haplotypes in Lake Wateree, South Carolina, suggests that these haplotypes will be particularly useful in detecting the coexistence of these subspecies.