Characterization of Prolamins from Meadow Grasses: Homology with those of Wheat, Barley and Rye

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Abstract
Prolamin fractions were extracted from seeds of five species of temperate meadow grasses. SDS-PAGE showed the presence of polypeptidcs with Mrs of 20–30000 in Phieum pratense and Dactylis glomerata, and between 30000 and 65 000 in Lolium perenne and in two species of Festuca (F. rubra and F. arundinacea). The fractions had broadly similar amino acid compositions with high glutamate+glutamine (29 to 35 mol %) and phenylalaninc (8 to 10 mol %), but there was some variation in the contents of prolinc (10 to 23 mol %) and several other amino acids including lysine (0.3 to 1.9 mol %). Automated Edman degradation of two gel filtration fractions from L perenne and one fraction each from the other four species showed single major N.terminal amino acid sequences. These were homologous with each other, and with the γ-typc prolamins of wheat, barley and rye.