THE EFFECT OF FRESH AND EXPERIMENTALLY MODIFIEDANTERIOR HYPOPHYSIS OF CATTLE ON MITOTICACTIVITY IN THE ADRENAL CORTEX OF THE GUINEA PIG1

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Abstract
Implantation of fresh cattle ant. hypophyseal lobes into immature [female] guinea pigs caused an increase in the number of mitoses in the adrenal cortex of these animals; injns. of acid extract of cattle gland had a similar effect. Immersion of cattle hypophysis in 95% alc., acetone, glycerin or 50% urea before implanta tion resulted in an increase in mitotic activity, which was not as high as that observed after implantation of fresh gland; treatment with combinations of urea and glycerin gave similar results. After immersion of cattle gland first in urea and then in 95% alc., or vice versa, there was either no or only a slight stimulation of mitotic proliferation in the adrenal cortex. 50% urea was shown to extract a part of the hormone, which was the cause of increased mitotic proliferations; this extractive action could be diminished or perhaps prevented by first treating the cattle gland with acetone or saturated ammonium sulfate before immersing it in 50% urea. The mitotic activity in the cortex of the adrenal gland was apparently a much more sensitive in dicator for the detn. of the effect of certain hormones than the change in wt. which took place in the gland under the influence of various agents.

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