A rapid cytokinin response assay inArabidopsisindicates a role for phospholipase D in cytokinin signalling
Open Access
- 4 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 515 (1-3) , 39-43
- https://doi.org/10.1016/s0014-5793(02)02415-8
Abstract
Seedlings of Arabidopsis thaliana harboring a fusion of the cytokinin‐responsive ARR5 gene promoter and the GUS reporter gene were used for a pharmacological approach to study cytokinin signal transduction. The assay was shown to be rapid, sensitive, dose‐dependent and highly specific for cytokinins, both adenine and phenylurea derivatives. Numerous inhibitors of known signalling pathways were tested and some were shown to suppress reporter gene induction. Particularly, primary alcohols that specifically inhibit phospholipase D (PLD) partially prevented cytokinin‐induced GUS activity and reduced the accumulation of ARR5 gene transcripts. This indicates a role for PLD early during cytokinin signalling.Keywords
This publication has 28 references indexed in Scilit:
- ARR1, a Transcription Factor for Genes Immediately Responsive to CytokininsScience, 2001
- Two-component circuitry in Arabidopsis cytokinin signal transductionNature, 2001
- Identification of CRE1 as a cytokinin receptor from ArabidopsisNature, 2001
- Characterization of the Response of the Arabidopsis Response Regulator Gene Family to CytokininPlant Physiology, 2000
- The Role of Phospholipase D in Signaling Cascades1Plant Physiology, 1999
- Two Genes with Similarity to Bacterial Response Regulators Are Rapidly and Specifically Induced by Cytokinin in ArabidopsisPlant Cell, 1998
- Cytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation.Plant Cell, 1992
- DNA Sequencing MarketsNature Biotechnology, 1990
- Inhibition of Cytokinin-Regulated Responses by Calmodulin-Binding CompoundsPlant Physiology, 1983
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976