Introducing genetically encoded aldehydes into proteins
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- 22 April 2007
- journal article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 3 (6) , 321-322
- https://doi.org/10.1038/nchembio878
Abstract
Methods for introducing bioorthogonal functionalities into proteins have become central to protein engineering efforts. Here we describe a method for the site-specific introduction of aldehyde groups into recombinant proteins using the 6-amino-acid consensus sequence recognized by the formylglycine-generating enzyme. This genetically encoded 'aldehyde tag' is no larger than a His(6) tag and can be exploited for numerous protein labeling applications.Keywords
This publication has 15 references indexed in Scilit:
- N‐Terminal Protein Modification through a Biomimetic Transamination ReactionAngewandte Chemie International Edition in English, 2006
- EXPANDING THE GENETIC CODEAnnual Review of Biophysics, 2006
- Site-specific labeling of cell surface proteins with biophysical probes using biotin ligaseNature Methods, 2005
- Non-canonical amino acids in protein engineeringPublished by Elsevier ,2003
- The human SUMF1 gene, required for posttranslational sulfatase modification, defines a new gene family which is conserved from pro- to eukaryotesGene, 2003
- The Multiple Sulfatase Deficiency Gene Encodes an Essential and Limiting Factor for the Activity of SulfatasesCell, 2003
- Multiple Sulfatase Deficiency Is Caused by Mutations in the Gene Encoding the Human Cα-Formylglycine Generating EnzymeCell, 2003
- Sequence determinants directing conversion of cysteine to formylglycine in eukaryotic sulfatasesThe EMBO Journal, 1999
- Specific Covalent Labeling of Recombinant Protein Molecules Inside Live CellsScience, 1998
- A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiencyCell, 1995