THE STIMULATION OF SUPEROXIDE ANION PRODUCTION IN GUINEA-PIG PERITONEAL-MACROPHAGES AND NEUTROPHILS BY PHORBOL-MYRISTATE ACETATE, OPSONIZED ZYMOSAN AND IGG2-CONTAINING SOLUBLE IMMUNE-COMPLEXES

  • 1 January 1983
    • journal article
    • research article
    • Vol. 48  (4) , 657-665
Abstract
The kinetics of superoxide anion production in guinea pig peritoneal macrophages and neutrophils were determined following in vitro stimulation with phorbol myristate acetate (PMA), opsonized zymosan (OZ) and soluble immune complexes of guinea pig IgG2 (SIC). Superoxide production was recorded as chemiluminescence (CL) arising from the reductive cleavage of lucigenin. With PMA, both macropahges and neutrophils displayed a 2-phase response consisting of a rapid initial burst of CL, which preceded ligand ingestion, followed by a plateau in the CL response which persisted for > 30 min. OZ induced a slow progressive increase in CL in both phagocytes which was consistent with the development of an oxidative burst concomitant with ingestion. The phagocytes differed in their responses to SIC, the macrophages displaying CL kinetics similar to those observed with PMA; the neutrophils responded in the manner observed with OZ. The relationship between disparity in the patterns of macrophage and neutrophil CL responses to SIC and differences in their expression of Fc receptors for IG2 is discussed.

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