Chemotaxis ofErwinia amylovora

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Abstract
Chemotaxis of E. amylovora is temperature- and pH-dependent with an optimum temperature range of 20-28.degree. C and pH 6-8. An incubation period of 30 min and a cell population not greater than 4.times. 107 cells/ml are optimal for chemotaxis studies. A medium consisting of 10-3 M EDTA, 10-3 M mannitol, 10-2 MgCl2 and 10-2 M potassium phosphate buffer at pH 7 was established for assays. Using these assay conditions, E. amylovora exhibits positive chemotaxis to apple nectar, to the organic acid fraction of apple nectar, to 1 amino acid (aspartate), to the organic acids fumarate, malate, malate, maleate, malonate, oxaloacetate and succinate, but not to any of the sugars tested. All attractants are dicarboxylic acids and responses of E. amylovora are uniformly inhibited by malate, suggesting a single chemoreceptor site for all the attractants. The chemoattractant response pattern of this strain and that of American Type Culture Collection strain 19382 of E. amylovora was identical.

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