Kinetically Controlled Enzyme-Catalyzed Synthesis of Kyotorphin
- 1 August 1987
- journal article
- research article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 15 (2) , 89-96
- https://doi.org/10.1007/bf02801310
Abstract
Regarding the α-chymotrypsin-catalyzed synthesis of Z-TyrArg-NH2, the effect on the reaction yield of the following experimental factors is discussed: DMF/buffer proportions, reaction temperature, and donor/nucleophile ratios. The experimental design shows that relatively better yields are obtained by increasing the reaction temperature and lowering the cosolvent proportion while maintaining a slight excess of nucleophile. The ascensional line reaches an optimal response surface very flat voided of a well-defined maximum.Keywords
This publication has 13 references indexed in Scilit:
- A putative met-enkephalin releaser, kyotorphin enhances intracellular Ca2+ in the synaptosomesBiochemical and Biophysical Research Communications, 1986
- A spectrophotometric assay for the characterization of the S′ subsite specificity of α-chymotrypsinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986
- Proteinase‐Catalyzed Synthesis of Peptide BondsPublished by Wiley ,1982
- Structure-Activity Relationships of Enkephalin-Like PeptidesAnnual Review of Pharmacology and Toxicology, 1980
- A novel analgesic dipeptide from bovine brain is a possible Met-enkephalin releaserNature, 1979
- Morphine-like analgesia by a new dipeptide, L-Tyrosyl-L-Arginine (kyotorphin) and its analogueEuropean Journal of Pharmacology, 1979
- Peptide. XXV. Synthese von Bradykinin‐Analoga und Partialsequenzen mit Tyrosin für Elektronenspinresonanz‐und FluoreszenzuntersuchungenJournal für Praktische Chemie, 1976
- Synthese von Heptapeptid‐Derivaten mit der Insulinsequenz B 14–20European Journal of Organic Chemistry, 1966
- Studies on Arginine Peptides. II. Synthesis of L-Arginyl-L-arginine and other N-Terminal Arginine DipeptidesJournal of the American Chemical Society, 1959
- Synthèse de la L‐histidyl‐L‐phénylalanyl‐L‐arginyl‐L‐tryptophanyl‐glycyl‐ϵ‐CBO‐L‐lysyl‐L‐prolyl‐L‐valylamideHelvetica Chimica Acta, 1958