Characterization of a cDNA clone coding for the beta chain of bovine fibrinogen.

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Abstract
Recombinant plasmids containing bovine cDNA [complementary DNA] were screened with a radiolabeled cDNA enriched for bovine fibrinogen. A number of plasmids containing cDNA for fibrinogen were identified by this assay. One plasmid, designated pBI.beta.1, contained a cDNA insert of 1372 base pairs. The sequence of the cDNA insert for this plasmid was then determined. It was shown to code for 424 amino acids of the .beta. chain of fibrinogen, starting with residue 44. This and other data made it possible to construct the complete amino acid sequence of the .beta. chain of the protein. Comparison of the amino acid sequence of the .beta. chain of bovine fibrinogen with the corresponding chain of the human molecule indicated that the 2 chains are > 80% homologous.