Actinomycin D induced DNase I hypersensitivity and asymmetric structure transmission in a DNA hexadecamer
Open Access
- 25 February 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (4) , 871-875
- https://doi.org/10.1093/nar/19.4.871
Abstract
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5′-to-3′ direction from the drug binding site. An inequivalent response of the two strands to a ligandinduced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.Keywords
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