Preparative Separation of Unsaturated Fatty Acids Esters by Centrifugal Partition Chromatography (CPC)

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Abstract
A novel method for separation of fatty acid ethyl esters by Centrifugal Partition Chromatography is reported in this paper. Data are first presented for laboratory-scale separations of fatty acid esters derived from cereal oil, primarily oleic, linoleic and linolenic acids, and from fish oil, containing, primarily, icosapentenoic and docosapentenoic acids. The method does not employ column packing resins; long-chain unsaturated fatty acids which are generally susceptible to oxidation on catalytic surfaces are readily purified by CPC. Scale-up design for industrial applications is discussed.
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