Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genes
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (20) , 6353-6362
- https://doi.org/10.1093/nar/10.20.6353
Abstract
A simple and efficient method for direct in. vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wfieat germ cell-free protein synthesizing systems.Keywords
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