Glutaminase in neurons and astrocytes cultured from mouse brain: Kinetic properties and effects of phosphate, glutamate, and ammonia
- 1 April 1988
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 13 (4) , 383-388
- https://doi.org/10.1007/bf00972489
Abstract
Phosphate activated glutaminase comprises two kinetically distinguishable enzyme forms in cultures of cerebellar granule cells, of cortical neurons and of astrocytes. Specific activity of glutaminase is higher in cultured neurons compared with astrocytes. Glutaminase is activated by phosphate in all cell types investigated, however, glutaminase in astrocytes reguires a much higher concentration of phosphate for half maximal activation. One of the products, glutamate, inhibits the enzyme strongly, whereas the other product ammonia has only a slight inhibitory action on the enzyme.This publication has 43 references indexed in Scilit:
- Pharmacologically distinct glutamate receptors on cerebellar granule cellsLife Sciences, 1986
- Uptake of GABA and nipecotic acid in astrocytes and neurons in primary cultures: Changes in the sodium coupling ratio during differentiationJournal of Neuroscience Research, 1986
- Selective culture of neurons from rat cerebral cortex: Morphological characterization, glutamate uptake and related enzymes during maturation in various culture mediaDevelopmental Brain Research, 1985
- Ontogenetic development of glutamate metabolizing enzymes in cultured cerebellar granule cells and in cerebellumin vivoNeurochemical Research, 1985
- Ion dependency of uptake and release of GABA and (RS)‐nipecotic acid studied in cultured mouse brain cortex neuronsJournal of Neuroscience Research, 1983
- Effect of Homo-?-Proline and Other Heterocyclic GAB A Analogues on GABA Uptake in Neurons and Astroglial Cells and on GABA Receptor BindingJournal of Neurochemistry, 1981
- Absence of preferential glutamine uptake into neurons — an indication of a net transfer of TCA constituents from nerve endings to astrocytes?Neuroscience Letters, 1980
- LOCALIZATION OF GLUTAMINASE IN THE RAT NEOSTRIATUMJournal of Neurochemistry, 1979
- PHOSPHATE ACTIVATED GLUTAMINASE ACTIVITY AND GLUTAMINE UPTAKE IN PRIMARY CULTURES OF ASTROCYTESJournal of Neurochemistry, 1979
- Rat cortical neurons in cell culture: Culture methods, cell morphology, electrophysiology, and synapse formationBrain Research, 1978