Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine

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Abstract
Extracellular superoxide dismutase was found in extraxylematic bark tissues and seedlings of Pinus sylvestris L. The isozymes were partially purified by infiltration and analysed by polyacrylamide gel electrophoresis. One of the major isoforms was purified to homogeneity and the N-terminal amino acid sequence was determined. The isoelectric point of these extracellular forms is distinctly higher than that of cellular superoxide dismutases. Furthermore, a novel type of superoxide dismutase with an isoelectric point higher than nine was detected in a crude needle extract.