Yellow Lupine Cyclophilin Transcripts Are Highly Accumulated in the Nodule Meristem Zone
Open Access
- 1 December 2001
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 14 (12) , 1384-1394
- https://doi.org/10.1094/mpmi.2001.14.12.1384
Abstract
Cyclophilin (CyP) is one of the enzymes that act as peptidylprolyl cis-trans isomerases (EC 5.2.1.8). The cDNA and an intronless gene coding for cytosolic CyP have been isolated from yellow lupine. The deduced amino acid sequence of the characterized open reading frame shows approximately 80% homology with cytosolic CyP from other organisms. Southern blots of genomic DNA indicate that there is a small family of genes for CyP-related genes in the yellow lupine genome. RNA blot analyses demonstrate that CyP genes are expressed in all plant organs. The amount of CyP transcripts is dramatically increased in root nodules. In situ hybridization experiments indicate that CyP transcripts are localized mainly in meristematic tissues, with the highest level observed in the nodule meristem zone. The promoter of the sequenced gene contains 5′ AAAGAT 3′ and AT-rich motifs that are characteristic for some nodulin promoters.Keywords
This publication has 63 references indexed in Scilit:
- Occurrence and role ofcis peptide bonds in protein structuresPublished by Elsevier ,2005
- Influence of proline residues on protein conformationPublished by Elsevier ,2004
- Medicago truncatula ENOD11: A Novel RPRP-Encoding Early Nodulin Gene Expressed During Mycorrhization in Arbuscule-Containing CellsMolecular Plant-Microbe Interactions®, 2001
- Legume Models Strut Their StuffMolecular Plant-Microbe Interactions®, 2001
- Summary of IS-MPMI Meeting, July 1999, AmsterdamMolecular Plant-Microbe Interactions®, 1999
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- A novel peptidyl‐prolyl cisltrans isomerase from Escherichia coliFEBS Letters, 1994
- Localization of repetitive proline-rich proteins in the extracellular matrix of pea root nodulesProtoplasma, 1994
- Site‐directed mutagenesis of the organ‐specific element in the soybean leghemoglobin Ibc3 gene promoterThe Plant Journal, 1993
- HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters.Plant Cell, 1990