A PROPOSED REVISION OF GEL DOUBLE DIFFUSION TEST FOR DETECTION OF MYCOBACTERIAL ANTIBODY

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Abstract
A revised method is given for performing the gel double diffusion precipitation test for mycobacterial antibody. The revision includes a new, more complete buffering of the gel diffusion reagents and a substitution of ion-agar No. 2 for the agar base, and recommends the use of at least four antigen concentrations for testing each serum specimen. Data are included to illustrate the value of using multiple antigen concentration. Some description and discussion are given of the appearance of positive bands of precipitate, and the manner in which the factors of time and change in relative antigen and antiserum concentrations may alter their appearance.

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