A Preliminary Investigation into the Antigenic Characters of Spore-Forming, Aerobic Bacteria

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Abstract
After injecting rabbits with Bacillus subtilis, Bac. mycoides, Bac. mesentericus, Bac. vulgatus, and Bac. cereus respectively antisera were obtained which gave complement-fixation and precipitation (of autolysate) with the bacterial type which was used in the immunization. Both in the immunizations and in the complement-fixation tests, cultures of about 12 hrs.'' standing, suspended in physiol. salt soln., were used. Spontaneous agglutination interfered with agglutination tests. The different spp. separated by their different biol. characters in the preliminary examinations now made seem to be serologically separated, at least, to some extent. The precipitation expts. with autolysates of a small number of strains suggest the presence of a specific antigen structure in the different types. The results of the complement-fixation tests with a fair number of strains point in the same direction even though the specificity does not appear-equally clear.

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