Induction of 8-azaguanine resistance and sister chromatid exchange in human lymphocytes exposed to mitomycin C and X rays in vitro
- 1 August 1981
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 292 (5824) , 601-605
- https://doi.org/10.1038/292601a0
Abstract
Indirect evidence implies that 8-azaguanine-resistant (AGr) lymphocytes in human peripheral blood are mutants associated with the loss of the hypoxanthine-guanine phosphoribosyltransferase (HPRT; EC 2.4.2.8) locus on the active X chromosome, the mutation frequency increasing linearly with age. AGr variants are readily induced in lymphocytes exposed to mitomycin C in vitro, their incidence correlating with induced sister chromatid exchanges (SCE). Although SCE events and the development of an AGr phenotype may reflect a common type of DNA damage, mitomycin C-induced AGr variants are not mutants but are suggested to be cells having a transcriptional block at the HPRT locus. AGr variants are also readily induced by X-rays in vitro, their incidence correlates closely with the incidence of aberrations induced in the X chromosome and they are considered to have a mutational origin.This publication has 25 references indexed in Scilit:
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