Molecular cloning and expression of Clostridium difficile toxin A in Escherichia coli K 12
Open Access
- 10 December 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 225 (1-2) , 82-86
- https://doi.org/10.1016/0014-5793(87)81135-3
Abstract
Clostridium difficile toxin A was purified to homogeneity and was used to raise monospecific antiserum in rabbits. A gene bank of C. difficile DNA in Escherichia coli was constructed by cloning Sau3A‐cleaved clostridial DNA fragments into the bacteriophage vector λEMBL3. Out of 4500 plaques screened with antitoxin A, 9 clones were positively identified. One of these clones λtA5 expressed a 235 kDa protein which exhibited a cytotonic effect on Chinese hamster ovary cells, and had the ability to haemagglutinate rabbit erythrocytes, both properties characteristic of toxin A. The size of the λtA5 insert DNA was 14.3 kb.Keywords
This publication has 25 references indexed in Scilit:
- Molecular cloning of Clostridium difficile toxin A gene fragment in λ gtllFEBS Letters, 1987
- Characterization of immunogenic p230 as the toxin A ofClostridium difficileFEMS Microbiology Letters, 1986
- Cytotonic effect ofClostridium difficileenterotoxin on Chinese hamster ovary cellsFEMS Microbiology Letters, 1986
- Purification and properties of toxins A and B ofClostridium difficileFEMS Microbiology Letters, 1986
- A new purification procedure for Clostridium difficile enterotoxinBiochemical and Biophysical Research Communications, 1984
- Clostridium difficile enterotoxin (toxin A): new resultsBiochemical Society Transactions, 1984
- Cytopathogenic Action of Clostridium Difficile ToxinsJournal of Toxicology: Toxin Reviews, 1984
- Presence ofClostridium difficile toxin in guinea pigs with penicillin-associated colitisMedical Microbiology and Immunology, 1981
- PSEUDOMEMBRANOUS COLITIS: PRESENCE OF CLOSTRIDIAL TOXINThe Lancet, 1977
- Clindamycin-Associated Colitis Due to a Toxin-Producing Species of Clostridium in HamstersThe Journal of Infectious Diseases, 1977