Staurosporine stimulates phospholipase D activation in human polymorphonuclear leukocytes
- 2 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 315 (1) , 33-37
- https://doi.org/10.1016/0014-5793(93)81127-l
Abstract
Treatment of 1-O-[3H]alkyl-2-acyl-phosphatidylcholine-prelabeled human polymorphonuclear leukocytes (PMNs) with Staurosporine (50 nM to 1 μM) induced a time- and concentration-dependent generation of tritiated phosphatidic acid (PA), reaching approximately 225% of the control value at 15–20 min. In the presence of ethanol, Staurosporine induced a production of phosphatidylethanol (PEt) reaching, 250% of control values, and partial inhibition of PA production, consistent with PLD activation. The amount of ether-linked acylglycerol (EAG) was weakly enhanced (29%) after 5 min of PMN treatment; longer treatment resulted in no significant EAG production, suggesting a possible late inhibition of PA hydrolase activity. Staurosporine concentrations that induced an elevation in PA completely depressed protein kinase C (PKC) activity in both soluble and particulate cell fractions, suggesting that PLD activation may occur independently from PKC activation. PLD may thus represent a potential cellular target for Staurosporine action.Keywords
This publication has 37 references indexed in Scilit:
- Antigen-induced phospholipase D activation in rat mast cells is independent of protein kinase CBiochemical and Biophysical Research Communications, 1991
- Inhibition of phosphatidic acid phosphohydrolase activity by sphingosineFEBS Letters, 1990
- Evidence for protein kinase C independent activation of phospholipase D by phorbol esters in lymphocytesBiochemical and Biophysical Research Communications, 1990
- Phosphatidylcholine hydrolysis stimulated by phorbol myristate acetate is mediated principally by phospholipase D in endothelial cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Phosphatidic acid and not diacylglycerol generated by phospholipase D is functionally linked to the activation of the NADPH oxidase by FMLP in human neutrophilsBiochemical and Biophysical Research Communications, 1990
- Staurosporine, a protein kinase inhibitor, up-regulates the stimulation of human neutrophil respiratory burst by N-formyl peptides and platelet activating factorBiochemical and Biophysical Research Communications, 1990
- Different effects of sphingosine, R59022 and anionic amphiphiles on two diacylglycerol kinase isozymes purified from porcine thymus cytosolFEBS Letters, 1989
- Differential activation by fMet‐Leu‐Phe and phorbol ester of a plasma membrane phosphatidylcholine‐specific phospholipase D in human neutrophilFEBS Letters, 1989
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959