Factors Influencing the Components of the Total Protonmotive Force in Mung Bean Mitochondria
- 1 February 1978
- journal article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 29 (1) , 1-12
- https://doi.org/10.1093/jxb/29.1.1-a
Abstract
The components of the protonmotive force (Δp) have been determined for mung bean mitochondria under various metabolic states using an ion distribution technique. Under state 4 conditions, a Δp of 180 mV is attained which is reduced to 150 mV during state 3 respiration. The addition of Ca2+ during state 4 conditions had no effect on either component of Δp, whilst an uncoupling agent such as 1799 completely collapsed both components. The hydrolysis of exogenous ATP generated a potential which was consistently 50 mV lower than that obtained under state 4 conditions. In the presence of 100 mM KCl, the value of Δp was 79 mV, the values of -ZΔpH and ΔΨ being 48 and 31 mV respectively.Keywords
This publication has 0 references indexed in Scilit: