METABOLISM OF 2,5-DIPHENYLOXAZOLE (PPO) BY TROUT LIVER MICROSOMAL MIXED-FUNCTION MONOOXYGENASE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 13  (3) , 439-447
Abstract
Trout liver microsomes metabolized PPO into at least 1 NaOH extractable fluorescing metabolite having a strong excitation peak at 345 nm and an emission peak at about 510 nm. In arbitrary fluorescent units (FU) Vmax was several fold higher in the trout than in the male rat hepatic microsomes. Km was 12.7 M, about twice as high as reported for mouse. Optimum assay conditions were established for the metabolism of PPO by trout liver microsomes. NADPH generating system was essential and the metabolism was strongly inhibited by .alpha.-napthoflavone, but much less markedly by SKF 525 A or metyrapone. Trout are very susceptible to chemical carcinogenesis by compounds such as PPO.

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