A New Method for the Conservation of Platelet Concentration During Extracorporeal Circulation

Publisher Website
Google Scholar
Abstract
Pluronic F-68, a non-ionic polyol, was investigated for its effect on platelet conservation, platelet function, release reaction, and red blood cell hemolysis during cardiopulmonary bypass (CPB). Three groups of ten dogs each underwent CPB utilizing both normothermia and hypothermia. The control group (bubble oxygenator) did not receive any Pluronic F-68. Experimental group I (bubble oxygenator) and group II (membrane oxygenator) received 2 mg of Pluronic F-68 per milliliter of priming fluid.After 10 minutes of normothermic CPB, the platelet concentration decreased significantly by 48%. The platelet concentration in group I significantly decreased by 38%, and in group II the decrease was a non-significant 8%. There was also an associated increase in the plasma concentration thromboxane B2 and platelet factor 4 in both the control group and group I. A significant increase in free plasma hemoglobin was observed in the control group. The increase in group I and II was not significant.During hypothermia (20°C), a significant change in platelets, thromboxane B2, and platelet factor 4 was observed in all groups. After re-establishing normothermia, platelet concentration remained significantly low at 48% in the control group, whereas the platelet concentration in group I increased to 80%, a decrease of 20%, and in group II increased to 92%, a decrease of 8%. Free plasma hemoglobin, platelet factor 4 and thromboxane B2 remained significantly elevated in the control and group I. There was no significant elevation in platelet factor 4, free plasma hemoglobin, and thromboxane B2 in group III.Pluronic F-68, an emulsifying agent, has therefore been shown to be effective in platelet preservation during CPB.

This publication has 0 references indexed in Scilit: