The use ofDaphnia magnafor detection of okadaic acid in mussel extracts
- 1 September 1993
- journal article
- research article
- Published by Taylor & Francis in Food Additives & Contaminants
- Vol. 10 (5) , 603-608
- https://doi.org/10.1080/02652039309374184
Abstract
Okadaic acid (OA), the main toxin responsible for diarrhetic shellfish poisoning (DSP) has toxicity for Daphnia magna (EC50= 15 ± 1.8 μg/l). A Daphnia bioassay was developed and used to analyse okadaic acid in mussel extracts. A linear correlation was found between OA concentration determined by the Daphnia bioassay and by HPLC assay (r = 0.85; p < 0.001). The Daphnia bioassay can measure OA levels 10 times below the threshold of the mouse bioassay method. It is an inexpensive sensitive tool which can be used in replacement of mouse bioassay for the screening of OA and some co‐extracting toxins in mussel extracts.Keywords
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