Collection and preservation of human placental blood

Publisher Website
Google Scholar
Abstract
High‐risk premature infants require red cell transfusions for anemia. Placental blood for autologous transfusions can be collected sterilely into citrate‐phosphate‐dextrose and stored at 4°C. During storage for 8 days, the placental red cell content of adenosine triphosphate remained normal. The 2,3,‐diphosphoglycerate concentration of cells stored 8 days declined sharply; however, the P50 value of the oxyhemoglobin dissociation curve declined to 24.4 ± 2.40 torr. During storage, placental blood underwent an exchange of extracellular Na+ and K+, but no change in glutathione content. Hemolysis was less than 1 percent. Bacteriologic and fungal cultures remained sterile. These in vitro studies suggest that human placental blood can be collected safely and preserved effectively for autologous red cell transfusion therapy.

This publication has 5 references indexed in Scilit: