Purification and Characterization of Human Plasma Zn-α2-Glycoprotein
- 1 December 1988
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 18 (4) , 413-430
- https://doi.org/10.1080/00327488808062541
Abstract
Zn-α2-glycoprotein (Znα2gp) was purified from fresh human plasma approximately 670-fold in a yield of 18% over the fractions from DEAE-Sephadex A-50 column chromatography. The purified protein was a glycoprotein with molecular weights of 56, 000 and 57, 000 on Superose and Sephadex G-150 column chromatographies and of 41, 000 and 42, 000 on nonreduced SDS-PAGE. Characterization, which included a determination of molecular weight, amino acid composition, amino terminus, and antigenicity, correlated well with known values previously reported for human Znα2gp.This publication has 15 references indexed in Scilit:
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