Monolayer-Controlled Substrate Selectivity Using Noncovalent Enzyme−Nanoparticle Conjugates
- 29 September 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 126 (42) , 13572-13573
- https://doi.org/10.1021/ja0461163
Abstract
Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.Keywords
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