Deoxyribopyrimidine triphosphatase activity specific for cells infected with herpes simplex virus type 1.
- 1 April 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (4) , 1872-1876
- https://doi.org/10.1073/pnas.77.4.1872
Abstract
Nuclei from baby hamster kidney cells infected with herpes simplex virus type 1 contain a virus-specific deoxyribonucleoside triphosphate degrading activity. The reaction proceeds at 4.degree. C and can thus be distinguished from host enzymes. Under these conditions the enzyme is specific for deoxyribopyrimidine triphosphates and catalyzes pyrophosphate cleavage to produce the monophosphates, dUTP being the best substrate followed by dCTP and dTTP. The appearance of the activity after infection parallels that of viral DNA-synthesis-related functions. Of a series of 8 temperature-sensitive mutants tested, 2 (tsD and tsK) exhibit significantly decreased triphosphatase levels after infection at nonpermissive temperature, whereas a viral deoxypyrimidine kinase-deficient mutant induced wild-type levels.This publication has 14 references indexed in Scilit:
- Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA.Journal of Biological Chemistry, 1978
- Cell-free synthesis of herpes simplex virus DNA; structure of the in vitro product and nucleolytic degradationBiochemistry, 1977
- Cell-free synthesis of herpes simplex virus DNA: conditions for optimal synthesisBiochemistry, 1977
- Ribonucleotide Reductase from Herpes Simplex Virus (Types 1 and 2) Infected and Uninfected KB Cells: Properties of the Partially Purified EnzymesJournal of General Virology, 1977
- Induction of deoxycytidine deaminase activity in mammalian cell lines by infection with herpes simplex virus type 1.Proceedings of the National Academy of Sciences, 1977
- Control of Protein Synthesis in Herpesvirus-infected Cells: Analysis of the Polypeptides Induced by Wild Type and Sixteen Temperature-sensitive Mutants of HSV strain 17Journal of General Virology, 1976
- Deoxyribonucleoside Triphosphate Pools in Herpes Simplex Type 1 Infected CellsJournal of General Virology, 1976
- Induction of Both Thymidine and Deoxycytidine Kinase Activity by Herpes VirusesJournal of General Virology, 1974
- Characterization of Herpes Simplex Virus-induced Deoxyribonucleic Acid PolymeraseJournal of Biological Chemistry, 1973
- Enzymatic synthesis of deoxyribonucleic acid. 28. The pyrophosphate exchange and pyrophosphorolysis reactions of deoxyribonucleic acid polymerase.1969