CHEMO-IMMUNOLOGICAL STUDIES ON CONJUGATED CARBOHYDRATE-PROTEINS

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Abstract
A method of synthesis of 2 hexosides from glucose and galactose and the coupling of these with proteins is described. From the 2 hexosides, horse serum globulin and crystallized egg albumin, 4 sugar-proteins were made: phenol b-gluco-side-azo-globulin, phenol b-glucoside-azo-albumin, phenol b-galactoside-azo-globulin, and phenol b-galacto-side-azo-albumin. Precipitin tests with serum of rabbits immunized by intravenous injection of the sugar-proteins showed that 2 antibodies are developed: one antiprotein was reactive only with the particular protein used in the coupling; the other anti-sugar-protein, with specificity dependent on the hexoside and independent of the protein radical, e.g., glucoside-albumin as well as glucoside-globulin, was precipitated by anti-glucoside-globulin serum, while galacto-albumin was not. Absorption of the antiprotein antibody failed to alter the titer of the anti-sugar-protein component; the presence of the former was ascribed to the excess of protein remaining after the coupling reaction. The uncoupled hexosides, themselves non-antigenic and un-precipitable in immune serum, specifically inhibited the reaction between the homologous sugar-protein and its specific antibody.

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