TRANSLATIONAL MOBILITY OF THE MEMBRANE INTERCALATED PARTICLES OF HUMAN ERYTHROCYTE GHOSTS
Open Access
- 1 June 1972
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 53 (3) , 777-787
- https://doi.org/10.1083/jcb.53.3.777
Abstract
This paper demonstrates the translational movement along the plane of the human erythrocyte ghost of the membrane particles exposed by freeze-fracture. The membrane particles can be aggregated by incubation of the ghosts in media with a pH in the vicinity of 5 5 or 3 5. The particles are disaggregated in neutral and alkaline media (pH 9 5) and also at pH 4.5 Aggregation of the particles at pH 5.5 is reversible, prevented by prefixation in glutaraldehyde and by media of high ionic strength. Particle aggregation occurs within 2-4 min. These results are consistent with the concept that the erythrocyte ghost membrane is a planar fluid domain formed by a bilayer membrane continuum which is interrupted by localized, yet mobile, proteic intercalations.Keywords
This publication has 19 references indexed in Scilit:
- The role of lipids in mitochondrial electron transfer and oxidative phosphorylationPublished by Elsevier ,2008
- A major protein which spans the human erythrocyte membraneJournal of Molecular Biology, 1971
- Proteins of the human erythrocyte membrane as modified by pronaseJournal of Molecular Biology, 1971
- Disposition of the major proteins in the isolated erythrocyte membrane. Proteolytic dissectionBiochemistry, 1971
- Microfilaments in Cellular and Developmental ProcessesScience, 1971
- Purification by Means of Detergents and Properties of Cytochrome b5 from Liver MicrosomesJournal of Biological Chemistry, 1968
- Protein conformations in cellular membranes.Proceedings of the National Academy of Sciences, 1966
- Fracture faces of frozen membranes.Proceedings of the National Academy of Sciences, 1966
- Calcium and magnesium binding properties of cell membrane materialsJournal of Cellular and Comparative Physiology, 1963
- Sodium, potassium and hydrogen ion binding characteristics of dog erythrocyte ghostsJournal of Cellular and Comparative Physiology, 1963