An enzyme-linked immunosorbent assay (ELISA) for detection of antibodies toEncephalitozoon cuniculiand its use in determination of infections in man

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Abstract
SUMMARY: An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies toEncephalitozoon cuniculiin man, using spores derived from tissue culture as antigen. Negligible cross-reactions were found with other microsporidia of vertebrate or of invertebrate origin and there was no cross-reaction withToxoplasma gondii, using ELISA, immunoperoxidase or immunofluorescence staining. A high prevalence of antibodies toE. cuniculiwas found in patients suffering from schistosomiasis, malaria and neurological and psychiatric disorders, but not in healthy individuals.